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Fluorescence Resonance
Energy Transfer (FRET) occurs when the emission of one fluorophore
overlaps the absorbance of a second fluorophore and is proportional
to their intermolecular distance (for review, see Wu and Brand,
Analytical Biochem, 218, 1-13, 1994). If the donor fluorophore is
excited with the appropriate wavelength of light, the result is
quenching of its emission and indirect excitation and emission from
the acceptor fluorophore.
Developed in the
laboratory of Dr. Roger Tsien at UCSD (Miyawaki et al, Nature, 388,
882-887, 1977), FRET using GFP spectral mutants provides the ability
to localize and monitor non-binding and molecular protein-protein
interaction in living cells.
Jobin Yvon Ltd offers the
high sensitivity Spex
FluoroMax
spectrofluorometer ideal for scanning FRET studies.
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Featured
Products |
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FluoroMax-3
The ideal
fluorometer for FRET studies |
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Filters
Our sister
company Glen Spectra has an extensive filter range for FRET studies |
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